Reactive species
Human,Mouse
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 167-267 of human
Neutrophil Elastase (ELANE) (P08246).
Dilution rate
WB 1:500-2000
Purification process
Affinity purification
Stockpile
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Other name
GE; NE; HLE; HNE; ELA2; SCN1; PMN-E; Neutrophil Elastase (ELANE)
Background
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to
elastin. Humans have six elastase genes which encode structurally similar proteins. The
encoded preproprotein is proteolytically processed to generate the active protease.
Following activation, this protease hydrolyzes proteins within specialized neutrophil
lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The
enzyme may play a role in degenerative and inflammatory diseases through proteolysis of
collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of
E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia.
Mutations in this gene are associated with cyclic neutropenia and severe congenital
neutropenia (SCN). This gene is present in a gene cluster on chromosome 19
