Reactive species
Human,Mouse,Rat
Protein name
Gasdermin domain-containing protein 1,Gasdermin-D,GSDMD,GSDMD-CT,GSDMD-NT,GSDMD_HUMAN
Immunogen
AA range: 1-100
Specificity
This antibody detects endogenous levels of GSDMD protein.
Constitute
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Dilution rate
WB 1:500-2000
Purification process
The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Function
Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms), CASP4, CASP5 or CASP8 relieves autoinhibition and is sufficient to initiate pyroptosis (PubMed:26375003, PubMed:29898893, PubMed:32109412). Cleavage by CASP1 and CASP4 is not strictly dependent on the consensus cleavage site on GSDMD but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32109412). Cleavage by CASP8 takes place following inactivation of MAP3K7/TAK1 by Yersinia toxin YopJ (By similarity). Cleavage at Asp-87 by CASP3 or CAPS7 inactivates the ability to mediate pyroptosis (PubMed:28392147, PubMed:28045099).
