Reactive species
Human;Mouse;Rat;
Applications
IHC;IF;ELISA
Gene Name
MYOD1 BHLHC1 MYF3 MYOD
Protein name
Myoblast determination protein 1 (Class C basic helix-loop-helix protein 1)
(bHLHc1) (Myogenic factor 3) (Myf-3)
Immunogen
Synthesized peptide derived from human MyoD1 AA range: 100-200
Specificity
This antibody detects endogenous levels of MyoD1 protein
Constitute
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Mouse, Monoclonal/IgG2b, kappa
Dilution rate
IHC 1:100-500. WB 1:500-2000. IF 1:100-500. ELISA 1:1000-5000
Purification process
Protein G
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Background
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix
family of transcription factors and the myogenic factors subfamily. It regulates
muscle cell differentiation by inducing cell cycle arrest, a prerequisite for
myogenic initiation. The protein is also involved in muscle regeneration. It
activates its own transcription which may stabilize commitment to myogenesis.
[provided by RefSeq, Jul 2008],
Function
function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts
to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with
and is inhibited by the twist protein. This interaction probably involves the basic
domains of both proteins.,online information:MyoD entry,PTM:Acetylated by a
complex containing EP300 and PCAF. The acetylation is essential to activate
target genes. Conversely, its deacetylation by SIRT1 inhibits its
function.,PTM:Ubiquitinated on the N-terminus; which is required for proteasomal
degradation.,similarity:Contains 1 basic helix-loop-helix (bHLH)
domain.,subunit:Efficient DNA binding requires dimerization with another bHLH
protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1.,
