Reactive species
Human;Mouse;Rat;Monkey
Applications
WB;IHC;IF;FCM;ELISA
Antibody type
Monoclonal antibodies
Immunogen
Purified recombinant fragment of human Prohibitin expressed in E. Coli.
Specificity
Prohibitin Monoclonal Antibody detects endogenous levels of Prohibitin protein.
Constitute
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification process
Affinity purification
Other name
PHB; Prohibitin
Background
prohibitin(PHB) Homo sapiens This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
Function
developmental stage:Levels of expression in fibroblasts decrease heterogeneously during cellular aging.,disease:Mutated in sporadic breast cancer.,function:Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.,induction:Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. Also induced following inhibition of mitochondrial protein synthesis by thiamphenicol.,similarity:Belongs to the prohibitin family.,subunit:Interacts with PHB2.,tissue specificity:Widely expressed in different tissues.,
