Reactive species
Human;Rat;Mouse;
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Diacylglycerol kinase delta
Immunogen
The antiserum was produced against synthesized peptide derived from human DGKD. AA range:41-90
Specificity
DGK-δ Polyclonal Antibody detects endogenous levels of DGK-δ protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
DGKD; KIAA0145; Diacylglycerol kinase delta; DAG kinase delta; 130 kDa diacylglycerol kinase; Diglyceride kinase delta; DGK-delta
Background
This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,enzyme regulation:Partially inhibited by phosphatidylserine.,function:May function as signaling molecule. Isoform 2 may be involved in cell growth and tumorigenesis.,PTM:Isoform 1 H domain is phosphorylated.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:The two isoforms are able to form homo- and hetero-oligomer structures (at least tetramers).,tissue specificity:Isoform 2 is ubiquitously expressed also in tumor tissues. Isoform 1 is expressed in ovary, spleen and some tumor-derived cells.,
