Reactive species
Human; Mouse;Rat
Applications
WB;ELISA;IHC
Antibody type
Polyclonal Antibody
Immunogen
Synthesized peptide derived from human LEO1 AA range: 159-209
Specificity
This antibody detects endogenous levels of LEO1 at Human/Mouse/Rat
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification process
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008],
Function
function:The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. May be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.,similarity:Belongs to the LEO1 family.,subunit:Component of the PAF1 complex, which consists of at least of CDC73, PAF1, LEO1 and CTR9. Interacts with CDC73.,tissue specificity:Highly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver.,
