Reactive species
Human; Mouse
Applications
WB;ELISA;IHC
Antibody type
Polyclonal Antibody
Immunogen
Synthesized peptide derived from human RIFK AA range: 82-132
Specificity
This antibody detects endogenous levels of RIFK at Human/Mouse
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification process
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009],
Function
catalytic activity:ATP + riboflavin = ADP + FMN.,cofactor:Zinc or magnesium.,function:Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).,pathway:Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.,subunit:Monomer.,tissue specificity:Detected in brain, placenta and urinary bladder.,