Reactive species
Human;Rat;Mouse;
Antibody type
Polyclonal Antibody
Immunogen
Synthesized peptide derived from human PCP
Specificity
This antibody detects endogenous levels of human PCP
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
IHC-p 1:50-200, WB 1:500-2000. IF 1:50-200
Purification process
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Other name
Lysosomal Pro-X carboxypeptidase (EC 3.4.16.2;Angiotensinase C;Lysosomal carboxypeptidase C;Proline carboxypeptidase;Prolylcarboxypeptidase;PRCP)
Background
This gene encodes a member of the peptidase S28 family of serine exopeptidases. The encoded preproprotein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. A pseudogene of this gene has been identified on chromosome 2. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [pr
Function
catalytic activity:Cleavage of a -Pro-|-Xaa bond to release a C-terminal amino acid.,function:Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.,similarity:Belongs to the peptidase S28 family.,subunit:Homodimer.,tissue specificity:Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.,
