Reactive species
Human;Mouse;Rat
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Gene Name
TXN TRDX TRX TRX1
Immunogen
Synthesized peptide derived from human Thioredoxin. at AA range: 52-101
Specificity
This antibody detects endogenous levels of Thioredoxin
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000. IF 1:50-200
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
Thioredoxin (Trx;ATL-derived factor;ADF;Surface-associated sulphydryl protein;SASP)
Background
The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
Function
function:ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).,function:Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity.,PTM:In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.,similarity:Belongs to the thioredoxin family.,similarity:Contains 1 thioredoxin domain.,subunit:Homodimer; di
