Reactive species
Human;Rat;Mouse;
Applications
IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Gene Name
ATG10 APG10L PP12616
Protein name
Ubiquitin-like-conjugating enzyme ATG10 (EC 6.3.2.-) (Autophagy-related protein 10) (APG10-like)
Immunogen
Synthetic peptide from human protein at AA range: 1-50
Specificity
The antibody detects endogenous ATG10
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
IHC-p 1:50-200, ELISA 1:10000-20000. IF 1:50-200
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
Ubiquitin-like-conjugating enzyme ATG10 (EC 6.3.2.-;Autophagy-related protein 10;APG10-like)
Background
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008],
Function
function:Plays a role in autophagy (By similarity). ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule.,similarity:Belongs to the ATG10 family.,subunit:Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7.,
