Reactive species
Human;Mouse
Applications
WB;ELISA;IHC
Antibody type
Polyclonal Antibody
Gene Name
MAPKAP1 MIP1 SIN1
Protein name
Sin1 (Thr86)
Immunogen
Synthesized phosho peptide around human Sin1 (Thr86)
Specificity
This antibody detects endogenous levels of Human Mouse Sin1 (phospho-Thr86)
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification process
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Other name
Target of rapamycin complex 2 subunit MAPKAP1 (TORC2 subunit MAPKAP1) (Mitogen-activated protein kinase 2-associated protein 1) (Stress-activated map kinase-interacting protein 1) (SAPK-interacting protein 1) (mSIN1)
Background
This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008],
Function
function:Essential component of the TORC2 complex, which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. Inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAs signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.,similarity:Belongs to the SIN1 family.,subunit:All isoforms except isoform 4 are able to be part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2.,tissue specificity:Ubiquitously expressed, with highest levels in heart and skeletal muscle.,
