Reactive species
Species independent
Immunogen
Recombinant Protein of GST-Tag
Specificity
The antibody detects C-terminal, internal, and N-terminal GST fusion proteins.
Constitute
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB: 1:5000-10000 ELISA 1:5000-20000
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Background
GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells.