Protein name
Protein lin-28 homolog A
Immunogen
Purified recombinant fragment of LIN-28 (aa93-209) expressed in E. Coli.
Specificity
LIN-28 Monoclonal Antibody detects endogenous levels of LIN-28 protein.
Constitute
Antibody are purified by protein G affinity chromatography. Liquid in 0.01M Phosphate buffer, pH 7.4 containing 0.03% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process
Affinity purification
Other name
LIN28A; CSDD1; LIN28; ZCCHC1; Protein lin-28 homolog A; Lin-28A; Zinc finger CCHC domain-containing protein 1
Background
This gene encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells. The encoded protein functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. This protein prevents the terminal processing of the LET7 family of microRNAs which are major regulators of cellular growth and differentiation. Aberrant expression of this gene is associated with cancer progression in multiple tissues. [provided by RefSeq, Sep 2015],
Function
developmental stage:Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.,domain:The CSD domain is required for function in muscle differentiation.,function:Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression.,induction:Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in
