Reactive species
Human;Mouse;Rat
Applications
WB;IHC;IF;ELISA
Protein name
Synaptotagmin-1/2
Immunogen
The antiserum was produced against synthesized peptide derived from human Synaptotagmin. AA range:176-225
Specificity
Synaptotagmin Polyclonal Antibody detects endogenous levels of Synaptotagmin protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
SYT1; SVP65; SYT; Synaptotagmin-1; Synaptotagmin I; SytI; p65; SYT2; Synaptotagmin-2; Synaptotagmin II; SytII
Background
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],
Function
cofactor:Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.,domain:The first C2 domain mediates Ca(2+)-dependent phospholipid binding.,domain:The second C2 domain mediates interaction with SV2A and STN2.,function:May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.,similarity:Belongs to the synaptotagmin family.,similarity:Contains 2 C2 domains.,subcellular location:Synaptic vesicles and chromaffin granules.,subunit:H
